Center of Excellence in Higher Education
The First Private University in Bangladesh

Dr. Md. Sazzad Hossen Toushik

Full Time Faculty

Assistant Professor

Ph.D. in Food Microbiology, Chung-Ang University, South Korea

M.Sc. in Microbiology, Norwegian University of Life Sciences, Norway

Phone: +880-2-55668200 Ext- 6841

Email: sazzad.toushik@northsouth.edu

Office: SAC# 820

 

Links        

      

Dr. Md. Sazzad Hossen Toushik is currently an Assistant Professor in the Department of Biochemistry & Microbiology at North South University. Previously, he worked as a Senior Lecturer at Stamford University, Bangladesh, and as a Postdoctoral Research Fellow (Professor Won-Bo Shim lab) at Gyeongsang National University, South Korea. Dr. Toushik earned his Ph.D. in Food Microbiology from Chung-Ang University (CAU), South Korea under the supervision of Professor Sang-Do Ha and received the Chung-Ang University Young Scientist Scholarship (CAYSS). During his Ph.D., he focused on identifying antimicrobial compounds in medicinal plants and probiotic bacteria against pathogenic bacterial biofilms in seafood. He holds a bachelor’s degree in Biotechnology and Genetic Engineering from Khulna University and an M.Sc. in Microbiology from the Norwegian University of Life Sciences (NMBU), Norway. Dr. Toushik's expertise lies in Food Safety, Biofilm, Molecular Biology, and Structural Biology, with his research published in prestigious international journals, and roles as a Review Editor in several peer-reviewed international journals.

Publications  

Key publications

1. Toushik SH*, Ashrafudoulla M. (2023). Recombinant expression in Bacillus megaterium and biochemical characterization of exo-mannered Glycosyl Hydrolase Family 43 α-L-Arabinofuranosidase from the Korean black goat rumen metagenome. Applied Microbiology. 3(4):1164-77. *Corresponding author. https://doi.org/10.3390/applmicrobiol3040080

2. Lee JE, Toushik SH*, Kim SA, Shim WB. (2023). Rapid detection of Shiga-toxin-producing Escherichia coli O157: H7 based on a colorimetric loop-mediated isothermal amplification (cLAMP) assay using a molecular beacon paired with HRPzyme. Analytical and Bioanalytical Chemistry, 1-12. *Co-first author. https://doi.org/10.1007/s00216-023-04803-7

3. Toushik SH*, Kim K, Park SH, Park JH, Ashrafudoulla M, Ulrich MS, Mizan MF, Hossain MI, Shim WB, Kang I, and Ha SD. (2023). Prophylactic efficacy of Lactobacillus curvatus B67-derived postbiotic and quercetin, separately and combined, against Listeria monocytogenes and Salmonella enterica ser. Typhimurium on processed meat sausage. Meat Science, 2:109065. *Co-first author. https://doi.org/10.1016/j.meatsci.2022.109065

4. Toushik SH, Roy A, Alam M, Rahman UH, Nath NK, Nahar S, Matubber B, Uddin MJ, and Roy PK. (2022). Pernicious attitude of microbial biofilms in agri-farm industries: acquisitions and challenges of existing antibiofilm approaches. Microorganisms, 10(12):2348. https://doi.org/10.3390/microorganisms10122348

5. Toushik SH, Park JH, Kim K, Ashrafudoulla M, Ulrich MSI, Mizan MFR, Roy PK, Shim WB, Kim YM, Park SH, and Ha SD. (2022). Antibiofilm efficacy of Leuconostoc mesenteroides J.27-derived postbiotic and food-grade essential oils against Vibrio parahaemolyticus, Pseudomonas aeruginosa, and Escherichia coli alone and in combination, and their application as a green preservative in the seafood industry. Food Research International, 111163. https://doi.org/10.1016/j.foodres.2022.111163

6. Toushik SH, Kim K, Ashrafudoulla M, Mizan MFR, Roy PK, Nahar S, Kim Y, and Ha SD. (2021). Korean kimchi-derived lactic acid bacteria inhibit foodborne pathogenic biofilm growth on seafood and food processing surface materials. Food Control, 108276. https://doi.org/10.1016/j.foodcont.2021.108276

7. Toushik SH, Mizan MFR, Hossain I, and Ha SD. (2020). Fighting with old foes: The pledge of microbe-derived biological agents to defeat mono-and mixed-bacterial biofilms concerning food industries. Trends in Food Science & Technology, 99:413-425. https://doi.org/10.1016/j.tifs.2020.03.019

8. Toushik SH*, Lee KT, Lee JS, and Kim KS. (2017). Functional applications of lignocellulolytic enzymes in the fruit and vegetable processing industries. Journal of Food Science, 82:585-593. *Co-first author.  https://doi.org/10.1111/1750-3841.13636

9. Lee KT, Toushik SH, Baek JY, Kim JE, Lee JS, and Kim KS. (2018). Metagenomic mining and functional characterization of a novel KG51 bifunctional cellulase/hemicellulase from black goat rumen. Journal of Agricultural and Food Chemistry, 66(34):9034- 41.  https://doi.org/10.1021/acs.jafc.8b01449

Conference papers, presentations, and workshops

  • Toushik SH, Lee JE, Kim SA, Kim DH, and Shim WB. Development of a colorimetric loop-mediated isothermal amplification assay based on a molecular beacon linked with HRP-mimicking sequence for the rapid detection of Escherichia coli O157:H7 in Korean beef. Poster at the KoSFoST International Symposium and Annual Meeting. The Korean Society of Food Science and Technology, Busan, Korea, July 6-8, 2022.
  • Toushik SH, Kim K, Park JH, Mizan MRF, and Ha SD. Combining postbiotic metabolites (Lactiplantibacillus plantarum [M.21]) with eugenol and thymol against pathogenic microbial biofilms on food-processing surfaces and MBEC™ biofilm device. Poster at the International Association for Food Protection (IAFP) Annual Meeting, Pittsburgh, PA USA, July 31-August 03, 2022.
  • Lee EJ, Choi ES, Han S, Hyun SW, Son JW, Toushik SH, Byun KW, and Ha SD. Inactivation of human coronavirus 229e by various disinfectants in suspension. Poster at the International Association for Food Protection (IAFP) Annual Meeting, Pittsburgh, PA USA, July 31-August 03, 2022.
  • Toushik SH and Ha SD. Inhibition of foodborne pathogens by Leuconostoc mesenteroides [J.27] postbiotic metabolites and essential oils. Oral presentation and Poster at the 6th Asia Pacific Symposium on Food Safety. The Korean Society of Food Hygiene and Safety, Jeju, Korea, November 10-12, 2021.
  • Toushik SH, Kim K, and Ha SD. Inhibition of foodborne pathogens (Vibrio parahaemolyticus, Pseudomonas aeruginosa, Escherichia coli) by kimchi-derived lactic acid bacteria in seafood model and food contact surfaces. Oral presentation and Poster at the Korean Society of Food Hygiene and Safety 35th Annual Meeting, Busan, Korea, November 5-6, 2020.
  • Toushik SH and Ha SD. Inhibition of pathogenic microorganisms in seafood by lactic acid bacteria. Workshop at Food Hygiene and Safety Group, Anseong, Korea, October 5-6, 2020.
  • Toushik SH, Hossain I, and Ha SD. Isolation, identification, and antimicrobial properties of bacteriocin-producing bacterium Lactobacillus plantarum (M.2) against Listeria monocytogenes. Poster at the Korean Society of Food Hygiene and Safety 34th Annual Meeting, Gyeongju, Korea, November 7-8, 2019.
  • Hossain I, Toushik SH, and Ha SD. Anti-biofilm effect of nisin alone and combined with food-grade oil components against Listeria monocytogenes on food and food contact surface. Poster at the Korean Society of Food Hygiene and Safety 34th Annual Meeting, Gyeongju, Korea, November 7-8, 2019.
  • Ashrafudoulla M, Mizan MFR, Byun KH, Hossain I, Nahar S, Toushik SH, and Ha SD. Antibacterial and antibiofilm mechanism of eugenol against Vibrio parahaemolyticus clinical and environmental isolates. Poster at the Korean Society of Food Hygiene and Safety 34th Annual Meeting, Gyeongju, Korea, November 7-8, 2019.
  • Ashrafudoulla M, Mizan MFR, Byun KH, Hossain I, Nahar S, Toushik SH, and Ha SD. Antibacterial and antibiofilm mechanism of eugenol against Vibrio parahaemolyticus clinical and environmental isolates. Poster at the International Association for Food Protection (IAFP) Annual Meeting, St, Louisville, KY, USA, July 21-24, 2019.
  • Hossain I, Mizan MFR, Toushik SH, Joo HJ, Lee JY, and Ha SD. Potential probiotic lactic acid bacteria isolated from kimchi: molecular characterization and inhibitory effects against Listeria monocytogenes biofilm on lettuce and stainless-steel surfaces. Poster at the Korean Society of Food Hygiene and Safety 33th Annual Meeting, Yeosu, Korea, October 11-12, 2018.

Book chapter

Toushik SH, and Ha SD (2021). Prophylactic character of Korean kimchi-derived lactic acid bacteria against foodborne pathogenic biofilms in seafood. In Molecular Characterization of Foodborne Pathogens, Environmental Effects of Biofilm Formation and Control Strategies. pp106-139, Eliva Press, Europe.

  • Guest Editor- Processes (Peer-reviewed open-access scientific journal), MDPI (2024)
  • Topical Advisory Panel Member- Foods (Peer-reviewed open-access scientific journal), MDPI (2024)
  • Topical Advisory Panel Member- Processes (Peer-reviewed open-access scientific journal), MDPI (2024)
  • Trainer- Biosafety Workshop, Institutional Biosafety Committee (IBC), Office of Research, North South University (2023)
  • Graduate Student Award- 6th Asia-Pacific Symposium on Food Safety (2021)
  • Young Scientist Award- 35th Korea Society of Food Hygiene and Safety Conference (2020)
  • BK21- Brain Korea 21 fellowship (2018-2020)
  • CAYSS-Chung-Ang University Young Scientist Scholarship (2018-2020)
  • CAYSS-Chung-Ang University Young Scientist Scholarship (2016-2018)

Ph.D. in Food Microbiology, Chung-Ang University, South Korea (2018-2022).

Dissertation Title: Mining of lactic acid bacteria from Korean kimchi and the pledge to inhibit foodborne pathogenic bacterial biofilms in aquatic food industries.

M.Sc. in Food Microbiology, Chung-Ang University, South Korea (2016-2018).

Dissertation Title: Recombinant expression in Bacillus megaterium and biochemical characterization of α-L-arabinofuranosidase (GH43) from black goat rumen metagenome.

M.Sc. in Microbiology, Norwegian University of Life Sciences, Norway (2011-2014). 

Dissertation Title: Purification and initial characterization of the H+/Mg2+ ATPase B from Salmonella Typhimurium.

B.Sc. in Biotechnology & Genetic Engineering, Khulna University, Bangladesh (2006-2010).

Dissertation Title: Study on health and sanitation status and microbial analysis of Aila-affected water of Batiaghata Upazila in Khulna district of Bangladesh.

07/2023 to till date     Assistant Professor, Dept. of Biochemistry & Microbiology, North South University

01/2023 to 06/2023     Senior Lecturer, Dept. of Microbiology, Stamford University Bangladesh

03/2022 to 12/2022     Post-doctoral Research Fellow, Dept. of Food Hygiene & Safety, Gyeongsang National University, KR 

09/2018 to 07/2020    Research Assistant, Dept. of Food Science & Technology, Chung-Ang University, KR

01/2013 to 07/2015    Research Technician, Biotechnology Centre of Oslo, University of Oslo (UiO), NOR

MIC 414    Food Microbiology & Quality Control

MIC 101/BIO 202    Basic Microbiology

BIO 103     Biology I

BIO 202L   Basic Microbiology Lab

BIO 103L    Biology I Lab

Member, Bangladesh Association of Biotechnology Graduates

Member, Korean Society of Food Hygiene and Safety

Member, Korean Society of Food Science and Technology

Member, International Students’ Union (ISU)-NMBU, Norway

Food Safety, Biofilm, Molecular Biology, and Structural Biology